MersV1, France, Analysis, bibRecord, 000405

Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.

Identifieur interne : 000405 ( France/Analysis ); précédent : 000404; suivant : 000406

Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.

Auteurs : J C Schulhof [France] ; D. Molko ; R. Teoule

Source :

Nucleic acids research [ 0305-1048 ] ; 1988.

RBID : pubmed:3340528

Descripteurs français

KwdFr :
- ADN (synthèse chimique), Indicateurs et réactifs, Oligodésoxyribonucléotides (synthèse chimique), Spectrométrie de masse, Thymine (analogues et dérivés).
MESH :
- analogues et dérivés : Thymine.
- synthèse chimique : ADN, Oligodésoxyribonucléotides.
- Indicateurs et réactifs, Spectrométrie de masse.

English descriptors

KwdEn :
- DNA (chemical synthesis), Indicators and Reagents, Mass Spectrometry, Oligodeoxyribonucleotides (chemical synthesis), Thymine (analogs & derivatives).
MESH :
- chemical , analogs & derivatives : Thymine.
- chemical , chemical synthesis : DNA, Oligodeoxyribonucleotides.
- chemical : Indicators and Reagents.
- Mass Spectrometry.

Abstract

5,6-Dihydrothymine is one of the most important products of base damage by gamma irradiation of DNA in anoxic conditions. This modified base is unstable in the deprotection conditions used for classical synthesis of oligonucleotides. For its incorporation in synthetic DNA fragments, a new set of amino protecting groups has been developed. The 5,6-dihydrothymidine phosphoramidite was successfully employed for the synthesis of two 14-mers and one 17-mer bearing this defect at positions corresponding to restriction enzymes sites. The presence of the modified base still intact in the oligonucleotides was evidenced by mass spectrometry in pyrolytic conditions.

DOI: 10.1093/nar/16.1.319
PubMed: 3340528

Affiliations:

France

Links to Exploration step

pubmed:3340528

Le document en format XML

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<author><name sortKey="Schulhof, J C" sort="Schulhof, J C" uniqKey="Schulhof J" first="J C" last="Schulhof">J C Schulhof</name>
<affiliation wicri:level="1"><nlm:affiliation>Département de Recherche Fondamentale de Grenoble, Centre d'Etudes, Nucléaires de Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
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<wicri:noRegion>Nucléaires de Grenoble</wicri:noRegion>
<wicri:noRegion>Nucléaires de Grenoble</wicri:noRegion>
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<author><name sortKey="Molko, D" sort="Molko, D" uniqKey="Molko D" first="D" last="Molko">D. Molko</name>
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<author><name sortKey="Teoule, R" sort="Teoule, R" uniqKey="Teoule R" first="R" last="Teoule">R. Teoule</name>
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<front><div type="abstract" xml:lang="en">5,6-Dihydrothymine is one of the most important products of base damage by gamma irradiation of DNA in anoxic conditions. This modified base is unstable in the deprotection conditions used for classical synthesis of oligonucleotides. For its incorporation in synthetic DNA fragments, a new set of amino protecting groups has been developed. The 5,6-dihydrothymidine phosphoramidite was successfully employed for the synthesis of two 14-mers and one 17-mer bearing this defect at positions corresponding to restriction enzymes sites. The presence of the modified base still intact in the oligonucleotides was evidenced by mass spectrometry in pyrolytic conditions.</div>
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Serveur d'exploration MERS

Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.

Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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